Microfluidics SP1.0 Platform

详细配置如下：

▲Microscope Axiovert 40:
Contrast option:Brightfield, Phase, Varel.
Objectives:10x LD A-Plan, 20x LD A-Plan, 32x CP-Achromat, 40x LD A-Plan
Eyepieces:E-PL 10x/20 Br., 10x/20 Br. foc.
Stage:Vena8 biochip compatible stage holder, Eppendorf tubes holder.

▲Mirus 1.0Nanopump
Specifications:Shear Stress Range of 0.05–20 dyne/cm2, Volumetric Flow Rates 100nL–20μL/minute (100μL syringe), Sample Volume Increments Freely adjustable
Linear Velocity Range of 10 μm/s to 10 cm/s; Flow Direction Reversible; Sample Volume Aspiration Accuracy ±1% ; Shear Stress Accuracy ±0.5%; Sample Volume Aspiration Precision < 1%.
Multichannel:16-Way manifold allowing individual addressing microchannels of Vena8 biochip.

▲Microscope Cageincubator:
Capabilities:Temperature control ± 0.10C, CO2 and humidity module.
Options:Temperature monitoring software module, black panels for use in fluorescent microscopy.

▲ Deltapix DP 200 USB 2.0camera
Specifications:1.3 Mega Pixels (1,280 x 1,024), Color CDD, 1/2" format, Pixel 5.2 μm x 5.2 μm, Resolution: 15 FPS @ 1,280 x 1,024 pixels; 24 FPS @ 1,204 x 768pixels; 37 FPS @ 800 x 600 pixels; 60 FPS @ 640 x 480 pixels. Dynamic Range > 60 dB.

▲Dell Optiplex 745 PCcomputer
Configuration:Intel Core2 processor; 1.86 GHz; 2GB RAM; ATI Radeon X1300PRO; Objective Imaging stage control hardware; 19” LCD monitor. Windows XP ProfessionalSP2; DeltaPix Viewer Software; FlowAssay and DucoCell software preinstalled.

▲FlowAssaySoftware.
Capabilities:Execution of preset protocols. Intuitive stepwise protocol interface with steps hierarchy and status control.

▲DucoCellsoftware
Specifications:Automatic Counting and Detection; Morphological parameters analyzed automatically(Area, diameter, form-factor, ellipticity, eccentricity,orientation, perimeter, asymmetry, cell centroid coordinates, elliptical axis); Cell sorting and filtering; Ability to analyze sub-populations based on inclusion/exclusion of morphologicalparameters. Data may be exported to Excel spreadsheet

incorporating automatic graph generation (e.g. % cell adhesion vs.shear stress). File Formats analyzed*.jpg, *.jpeg, *.tif, *.tiff, *.bmp, *.gif, *.png.

▲Vena8 biochipspack of 10
Specifications:Minimum Sample Volume 3 μL; Maximum Sample Volume 100 μL; Shear Stress Range of 0.05– 20 dyne/cm2, steps of 0.05dyne/cm2. Number of channels perbiochip is 8; Volume of each channel is 0.8μL. Dead volume at input port is 0.1 μL. Vena8 biochip dimensions are 400 μm (W) x 100 μm (D) x 20 mm(L).